Inverted high-speed live imaging microscope. Suited for fluorescence imaging of live samples mounted in MatTek dishes. Applications include TIRF imaging, FLIM imaging, measuring FRET interactions, rapid acquisition (60Hz+) and FRAP experiments.

Features Include:

  • TIRF (total internal reflection fluorescence)
  • FRET (Förster resonance energy transfer)
  • Photoablation laser for FRAP (488nm)
  • Simultaneous dual colour imaging (GFP/RFP or CFP/YFP)
  • CO2 and temperature incubation controls for live cell imaging
  • Fully motorised X-Y-Z stage
  • Z-stacks
  • Tiled imaging
  • Mark and Find
  • Automated image capture experiments

Objectives:

  • Air: 10x (0.45), 20x (0.8), 40x (0.75)
  • Oil: 100x (1.46)

Fluorescent Filter Sets:

  • CFP
  • YFP
  • GFP
  • RFP
  • CFP/YFP
  • GFP/RFP
  • Additional filters for DAPI and Alexa 647

Fluorescence Illumination:

  • High-speed DG4 Xenon Lamp

Lasers:

  • 405nm
  • 445nm
    • CFP
  • 488nm
    • GFP
  • 515nm
    • YFP
  • 561nm
    • RFP / mCherry

Instructions

Marianas TIRF Microscope Guide