Queensland Brain Institute

Epifluorescence Microscopes: Axio Observer Indigo (616) - Inverted

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Inverted fluorescent microscope fitted with a monochrome camera. Suited for fluorescence and bright field imaging of live cells in flasks, dishes or glass-bottomed MatTek dishes.

Features Include:

  • CO2 and temperature incubation controls for live cell imaging
  • Fully motorised X-Y-Z stage
  • Z-stack imaging
  • MosaiX tiled imaging
  • Mark and Find for fully automated image capture and high-throughput experiments
  • ApoTome optical sectioning
  • Objectives have long working distances (good for plastic dishes)
  • Different stage inserts for small dishes, multi-well plates and flasks
  • Heated stage inserts for MatTek dishes and 24well plates
  • DIC
  • plasDIC for DIC imaging through plastic

Objectives:

  • Air:
    • 2.5x 0.16 NA / 18.5mm WD / 2.58 μm/pixel,
    • 10x 0.3 NA / 5.2mm WD / 0.645 μm/pixel,
    • 20x 0.8 NA / 0.55mm WD / 0.323 μm/pixel,
    • 20x 0.4 NA / 7.9mm WD / 0.323 μm/pixel,
    • 40x 0.6 NA / 2.9mm WD / 0.161 μm/pixel
  • Glycerine:
    • 63x 1.3 NA / 0.17mm WD / 0.102 μm/pixel

Fluorescent Filter Sets For:

  • DAPI (FS#49) BP335-383/BS395/BP 420-470
  • GFP / Alexa 488 (FS#38) BP450-490/BS495/BP500-550
  • Cy3 / Alexa 546/555/568 (FS#43) BP533-558/BS570/BP570-640
  • mCherry / Alexa 594 (FS#43) BP533-558/BS570/BP570-640
  • Cy5 / Alexa 633/647 (FS#50) BP625-655/BS660/BP665-715

Fluorescent Illumination:

  • Colibri LEDs – 365nm (DAPI), 470nm (GFP), 590nm (mCherry/Alexa594), 625nm (Cy5/Alexa647)

Illumination Power:

  • Xenon HXP:
    • 405nm (1.7mW),
    • 488nm (9.49mW),
    • 568nm (5.9mW),
    • 647nm (14.73mW)

Instructions

Guide to using Axio Imagers and Observers

Guide to High-throughput Imaging

 

When including this microscope in your methods:

Cells/tissue/specimens were imaged on an Axio Observer Z1 inverted fluorescence microscope (Carl Zeiss Pty Ltd) fitted with a full-enclosure incubator with CO2 and temperature control for live-cell imaging, an Axiocam 702 monochrome high-speed camera (Carl Zeiss), and a 20x/0.8 NA Plan-Apochromat objective (or other – please refer to objectives list) (Carl Zeiss). Image acquisition was performed using ZEN software (Carl Zeiss).