The Abbelight SAFe 360 is an inverted single-molecule localisation microscope (SMLM)  that features the SAFe Light excitation module with Adaptive Scanning for Tuneable Excitation Regions (ASTER), a hybrid scanning and wide-field illumination technique for TIRF and 2D/3D SMLM that offers a large and homogeneously illuminated field of view of 150 x 150 um^2. Excitation modes include epi-, HILO (highly inclined and laminated optical sheet), and TIRF.

The microscope is built around a Nikon ECLIPSE Ti2 body and is controlled by NEO software and equipped with two identical Hamamatsu ORCA-Fusion BT sCMOS cameras for simultaneous dual-channel acquisition.

For more information on these techniques and to request training  please use the PPMS booking system.


  • Spectral demixing with far red fluorophores (Alexa Fluor 647, CF®660,  and CF®680 recommended)
  • 2 x Hamamatsu ORCA-Fusion BT sCMOS cameras (95 % quantum efficiency)
  • Mad City Labs motorised xyz stage incorporating a 100-µm piezo z-drive
  • Nikon Perfect Focus System for focus stabilisation
  • Epi-, HILO, and TIRF illumination
  • Okolab stage-top incubator with temperature and CO2 control
  • SMART Flow fluidic system for liquid handling


  • CFI Apo TIRF 100XC/1.49 NA oil-immersion


  • 405 nm (60 mW)
  • 488 nm (120 mW)
  • 561 nm (240 mW)
  • 640 nm (600 mW)

To cite the use of the Abbelight SAFe 360 SMLM in a manuscript, please refer to the following suggestion:

“Image acquisition was performed using a Nikon CFI Apo TIRF 100x/1.49 NA oil-immersion objective on a single-molecule localisation microscope (SAFe 360; Abbelight, France) built around a Nikon ECLIPSE Ti2 body and equipped with two ORCA-Fusion BT sCMOS cameras (Hamamatsu Photonics K.K., Japan) and controlled by Abbelight NEO software.”



Guide to using the Abbelight SAFe 360 SMLM