Epifluorescence Microscopes: Axio Imager Green (414)

Upright fluorescent microscope fitted with both monochrome and colour cameras. Suited for fluorescence and bright field imaging of fixed samples on slides.

Features Include:

• Fully motorised X-Y-Z stage
• Z-stacks
• Mosaix tiling
• Mark and Find
• Fully automated image capture experiments
• ApoTome optical sectioning
• DIC

Objectives:

• Air:
  • 5x 0.16 NA / 18.5mm WD / 1.29 μm/pixel,
  • 10x  0.45 NA / 2mm WD / 0.645 μm/pixel,
  • 20x 0.8 NA / 0.55mm WD / 0.323 μm/pixel,
  • 40x 0.75 NA / 0.71mm WD /0.161 μm/pixel
• Oil:
  • 63x 1.4 NA / 0.19mm WD / 0.102 μm/pixel
  • 100x 1.3 NA / 0.2mm WD / 0.065 μm/pixel

Fluorescent Filter Sets For:

• DAPI (FS#49) BP335-383/BS395/BP 420-470
• GFP / Alexa 488 (FS#44) BP455-495/BS500/BP505-555
• Cy3 / Alexa 546/555/568 (FS#43) BP533-558/BS570/BP570-640
• DAPI / BFP / GFP / Alexa 488 / HcRed / mCherry / Alexa 594 (FS#62HE)
  • BP450-390/BS395/BP402-448
  • BP460-488/BS495/BP500-557
  • BP567-602/BS610/LP615
• Cy5 / Alexa 633/647 (FS#50) BP625-655/BS660/BP665-715
• CFP (FS#47) BP426-446/BS455/ BP460-500
• YFP (FS#46) BP490-510/BS515/BP520-550

Fluorescent Illumination:

• Xenon HXP
• CoolLED pE-300 LEDs – 365nm (DAPI), 460nm (GFP/Alexa488), GYR (525-660nm) (Alexa546/555/568/594/mCherry/Cy5/Alexa647)

Illumination Power:

• Xenon HXP: 405nm (3.99mW), 488nm (12.34mW), 568nm (14.22mW), 647nm (4.51mW)

Instructions

Guide to using Axio Imagers and Observers

When including this microscope in your methods:

Cells/tissue/specimens were imaged on an Axio Imager Z1 upright fluorescence microscope (Carl Zeiss Pty Ltd) fitted with an Axiocam 506 mono^{(or 512 colour)} camera (Carl Zeiss), and a 20x/0.8 NA Plan-Apochromat objective ^{(or other – please refer to objectives list)} (Carl Zeiss). Image acquisition was performed using ZEN software (Carl Zeiss).