Epifluorescence Microscopes: Axio Imager Orange (616)

Upright fluorescent microscope fitted with both monochrome and colour cameras. Suited for fluorescence and bright field imaging of fixed samples on slides.

Features Include:

• Fully motorised X-Y-Z stage
• Z-stacks
• Mosaix tiling
• Mark and Find
• Fully automated image capture experiments
• ApoTome optical sectioning

Objectives:

• Air:
  • 2.5x 0.075 NA / 9.5mm WD / 2.58 μm/pixel,
  • 5x 0.15 NA / 13.6mm WD / 1.29 μm/pixel,
  • 10x 0.3 NA / 0.52mm WD / 0.645 μm/pixel,
  • 20x 0.5 NA / 2.0mm WD / 0.323 μm/pixel,
  • 40x 0.95 NA / 0.25µm WD / 0.161 μm/pixel

• Glyc/Water:
  • 63x  1.3 NA / 0.17mm WD / 0.102 μm/pixel

Fluorescent Filter Sets For:

• DAPI (FS#49) BP335-383/BS395/BP 420-470
• GFP / Alexa 488 (FS#44) BP455-495/BS500/BP505-555
• Cy3 / Alexa 546/555/568 (FS#43) BP533-558/BS570/BP570-640
• Cy5 / Alexa 633/647 (FS#50) BP625-655/BS660/BP665-715
• DAPI / BFP / GFP / Alexa 488 / HcRed / mCherry / Alexa 594 (FS#62HE)
  • BP450-390/BS395/BP402-448
  • BP460-488/BS495/BP500-557
  • BP567-602/BS610/LP615

Fluorescent Illumination:

• Xenon HXP
• Colibri LEDs – 365nm (DAPI), 470nm (GFP), 590nm (mCherry/Alexa594), 625nm (Cy5/Alexa647)

Illumination Power:

• Xenon HXP: 405nm (1.92mW), 488nm (6.20mW), 568nm (7.67mW), 647nm (3.56mW)
• Colibri LEDs:  405nm (5.01mW), 488nm (12.80mW), 590nm (3.38mW), 647nm (15.38mW)

Instructions

Guide to using Axio Imagers and Observers

When including this microscope in your methods:

Cells/tissue/specimens were imaged on an Axio Imager Z1 upright fluorescence microscope (Carl Zeiss Pty Ltd) fitted with an Axiocam MRm^{(or MRc)} camera (Carl Zeiss), and a 20x/0.8 NA Plan-Apochromat objective ^{(or other – please refer to objectives list)} (Carl Zeiss). Image acquisition was performed using ZEN software (Carl Zeiss).