Confocal Microscopes: LSM 510 META (Level 5 Imaging Suite)
Inverted point-scanning laser confocal microscope with spectral detection. Ideal for imaging live cells or tissue.
Features Include:
- CO2 and temperature incubation for live cells and tissue
- Fully motorised X-Y-Z stage
- Z-stacks
- Tiled imaging
- Mark and Find
- Automated image capture experiments
- Online fingerprinting – for imaging with multiple spectrally overlapping fluorophores and removing background fluorescence
Objectives:
- Air:
- 10x 0.45 NA / 2000µm WD / 0.439 μm/pixel,
- 20x 0.8 NA / 550µm WD / 0.22 μm/pixel,
- Water:
- 40x 1.1 NA / 620µm WD / 0.11 μm/pixel,
- Oil:
- 63x 1.4 NA / 190µm WD / 0.19 μm/pixel
Fluorescent Filter Sets (for checking sample with oculars):
- DAPI
- GFP / Alexa 488
- Cy3 / Alexa 546/555/568
- Cy5 / Alexa 633/647
Lasers:
- Blue laser diode (405nm)
- DAPI
- Argon laser (458/477/488/514nm)
- GFP / YFP / FITC / Alexa488
- Melles Griot DPSS 561nm
- Cy3 / Alexa 546/555/568 / Alexa 594 / mCherry
- HeNe 633nm
- Cy5 / Alexa647
Laser Power:
Measurement at BFP
- 405nm (1.51 mW),
- 458nm (0.06 mW),
- 488nm (0.9 mW),
- 514nm (0.3 mW),
- 561nm (1.2 mW),
- 633nm (1.1 mW)
Instructions