TIRF Microscope: Marianas TIRF FRET (Level 5 Imaging Suite)
Inverted high-speed live imaging microscope. Suited for fluorescence imaging of live samples mounted in MatTek dishes. Applications include TIRF imaging, measuring FRET interactions, and rapid acquisition (60Hz+) experiments.
Features Include:
- TIRF (total internal reflection fluorescence)
- FRET (Förster resonance energy transfer)
- Simultaneous dual colour imaging (GFP/RFP or CFP/YFP)
- CO2 and temperature incubation controls for live cell imaging
- Fully motorised X-Y-Z stage
- Z-stacks
- Tiled imaging
- Mark and Find
- Automated image capture experiments
Objectives:
- Air: 10x (0.45), 20x (0.8), 40x (0.75)
- Oil: 100x (1.46)
Fluorescent Filter Sets:
- CFP
- YFP
- GFP
- RFP
- CFP/YFP
- GFP/RFP
- Additional filters for DAPI and Alexa 647
Fluorescence Illumination:
- HXP 120 Mercury Lamp
Lasers:
- 405nm
- 445nm
- CFP
- 488nm
- GFP
- 515nm
- YFP
- 561nm
- RFP / mCherry
Instructions