Inverted fluorescent microscope fitted with a monochrome camera. Suited for fluorescence and bright field imaging of cells in flasks and dishes.

Features Include:

  • Z-stacks
  • ApoTome optical sectioning
  • DIC (will not work with plastic)

Objectives:

  • Air:
    • 10x 0.3 NA / 5.5mm WD /
    • 20x 0.4 NA / 7.9mm WD /
    • 40x 0.6 NA / 2.9mm WD /
  • Oil:
    •  63x 1.4 NA / 0.19mm WD /
    • 40x 1.3 NA / 0.21mm WD /

Fluorescent Filter Sets For:

  • DAPI (FS#49)
  • GFP / Alexa 488 (FS#38)
  • Cy3 / Alexa 546/555/568 (FS#43)
  • mCherry / Alexa594 (FS#62HE)

Fluorescent Illumination:

  • Xenon HXP
  • Colibri LEDs – 365nm (DAPI), 470nm (GFP), 530nm (Cy3, Alexa555), 590nm (mCherry / Alexa594).

Cameras:

  • Andor Luca-R EMCCD

Instructions

Guide to using Axio Imagers and Observers

 

When including this microscope in your methods:

Cells/tissue/specimens were imaged on an Axio Observer Z1 inverted fluorescence microscope (Carl Zeiss Pty Ltd) fitted with a Luca-R EMCCD camera (Andor), and a 20x/0.8 NA Plan-Apochromat objective (or other – please refer to objectives list) (Carl Zeiss). Image acquisition was performed using ZEN software (Carl Zeiss).