Epifluorescence Microscopes: Axio Observer Yellow (CRISP) (Ritchie 3b) - Inverted
Inverted fluorescent microscope fitted with a monochrome camera. Suited for fluorescence and bright field imaging of cells in flasks and dishes.
Features Include:
- Z-stacks
- ApoTome optical sectioning
- DIC (will not work with plastic)
Objectives:
- Air:
-
- 10x 0.3 NA / 5.5mm WD /
- 20x 0.4 NA / 7.9mm WD /
- 40x 0.6 NA / 2.9mm WD /
- Oil:
- 63x 1.4 NA / 0.19mm WD /
- 40x 1.3 NA / 0.21mm WD /
Fluorescent Filter Sets For:
- DAPI (FS#49)
- GFP / Alexa 488 (FS#38)
- Cy3 / Alexa 546/555/568 (FS#43)
- mCherry / Alexa594 (FS#62HE)
Fluorescent Illumination:
- Xenon HXP
- Colibri LEDs – 365nm (DAPI), 470nm (GFP), 530nm (Cy3, Alexa555), 590nm (mCherry / Alexa594).
Cameras:
- Andor Luca-R EMCCD
Instructions
Guide to using Axio Imagers and Observers
When including this microscope in your methods:
Cells/tissue/specimens were imaged on an Axio Observer Z1 inverted fluorescence microscope (Carl Zeiss Pty Ltd) fitted with a Luca-R EMCCD camera (Andor), and a 20x/0.8 NA Plan-Apochromat objective (or other – please refer to objectives list) (Carl Zeiss). Image acquisition was performed using ZEN software (Carl Zeiss).