Epifluorescence Microscopes: Axio Imager Azure (618)

Upright fluorescent microscope fitted with both monochrome and colour cameras. Suited for fluorescence and bright field imaging of fixed samples on slides.

Features Include:

• Fully motorised X-Y-Z stage
• Z-stacks
• Mosaix tiling
• Mark and Find
• Fully automated image capture experiments
• ApoTome optical sectioning

Objectives:

• Air:
  • 2.5x 0.075 NA / 9.5mm WD / 2.58 μm/pixel,
  • 5x 0.15 NA / 13.6mm WD / 1.29 μm/pixel,
  • 10x 0.3 NA / 0.52mm WD / 0.645 μm/pixel,
  • 20x 0.8 NA / 0.55mm WD / 0.323 μm/pixel,
  • 40x 0.95 NA / 0.25µm WD / 0.161 μm/pixel

• Oil:
  • 63x 1.4 NA / 0.19mm WD / 0.102 μm/pixel

Fluorescent Filter Sets For:

• DAPI (FS#49) BP335-383/BS395/BP 420-470
• GFP / Alexa 488 (FS#44) BP455-495/BS500/BP505-555
• Cy3 / Alexa 546/555/568 (FS#43) BP533-558/BS570/BP570-640
• mCherry / Alexa 594 (FS#43) BP533-558/BS570/BP570-640
• Cy5 / Alexa 633/647 (FS#50) BP625-655/BS660/BP665-715

Fluorescent Illumination:

• Xenon HXP
• CoolLED pE-300 LEDs – 365nm (DAPI), 460nm (GFP/Alexa488), GYR (525-660nm) (Alexa546/555/568/594/mCherry/Cy5/Alexa647)

Illumination Power:

• Xenon HXP: 405nm (8.92mW), 488nm (6.39mW), 568nm (19.57mW), 647nm (5.83mW)

Instructions

Guide to using Axio Imagers and Observers

When including this microscope in your methods:

Cells/tissue/specimens were imaged on an Axio Imager Z1 upright fluorescence microscope (Carl Zeiss Pty Ltd) fitted with an Axiocam 506 mono^{(or 512 colour)} camera (Carl Zeiss), and a 20x/0.8 NA Plan-Apochromat objective ^{(or other – please refer to objectives list)} (Carl Zeiss). Image acquisition was performed using ZEN software (Carl Zeiss).