Epifluorescence Microscopes: Nikon Ti Eclipse Amber (Ritchie 3b)

Amber is an inverted fluorescence/bright-field wide-field microscope built on a Nikon Eclipse Ti body. It is fitted with a Nikon DS-Qi1Mc monochrome camera, an (from the Nikon website) ``ultra high-quality monochrome, cooled digital microscope camera geared for high-speed, high-sensitivity applications'' (low-noise electronics + high-quantum efficiency detector, ideal for fluorescence imaging).

The microscope is suited for fluorescence and bright-field imaging (including DIC) of live cells in flasks, dishes or glass-bottomed MatTek dishes.

Features Include:

• Z-stack imaging
• Objectives have long working distances (good for plastic dishes)
• Low magnification objectives for human tissue sections
• DIC

Objectives:

• Air:
  • Plan Apo Lambda 10x/0.45 NA air, DIC N1, WD 4.0 mm
  • S Plan Fluor ELWD 20x/0.45 NA air, Ph1, WD 8.2-6.9 mm
  • Plan Apo VC 20x/0.75 NA air, DIC N2, WD 1.0 mm
  • Plan Fluor 40x/1.30 NA oil, WD 240 um
  • Plan Apo Lambda 60x/1.40 NA oil, DIC N2, WD 130 um

Fluorescent Filter Sets For:

• DAPI
• GFP / Alexa 488
• Cy3 / Alexa 546/555/568
• Cy5 / Alexa 633/647

Fluorescent Illumination Source:

• Nikon Intensilight