Advanced Microscopy Facility
The QBI Advanced Microimaging and Analysis Facility is built around 24 high-end instruments. These include specialised microscope systems capable of super-resolution imaging, automated imaging, optical sectioning, tiled imaging of large areas and neuronal morphology analysis. Five confocal systems allow high-resolution imaging (Zeiss LSM710 and LSM510 METAs), high-speed live imaging (3i Yokogawa W1 SDC) and immersion microscopy (LSM PASCAL). Super-resolution imaging techniques, including Structured Illumination Microscopy (SIM) and single molecule imaging techniques (STORM/PALM), are made possible on a Zeiss ELYRA PS and Roper Ilas2 instrument. Stereology, slide-scanning, calcium imaging, TIRF, FLIM, FRET, FRAP, 2-photon imaging and a variety of other imaging techniques are also possible within the facility.
In addition to state-of-the-art instrumentation, a dedicated team of experienced staff provide training, support and experimental advice to researchers. This combination provides QBI researchers and collaborators with the tools to undertake diverse studies, from tissue mapping and 3-D reconstruction of neuronal populations through to rapid imaging of neural activity and intracellular trafficking.
- Information and advice on imaging techniques and experiment design.
- Instrument training: from basic use through to advanced techniques
- Training in post image analysis – including Imaris, Neurolucida, CellProfiler, Deconvolution, Image/FIJI
- Ongoing tailored advice and support for imaging projects
- Brightfield and mullti-channel fluorescence microscopy.
- Laser scanning confocal microscopy, including spectral imaging and multi-positional imaging.
- Rapid spinning disk confocal imaging for experiments in tissue and cells
- Super-resolution imaging using STORM/PALM techniques and structured illumination (SIM)
- 2-photon confocal imaging for tissue imaging and nano-scissor experiments.
- Long-term (48hr+) live imaging in brightfield and fluorescence.
- Rapid-acquisition live imaging for intracellular trafficking and calcium imaging.
- TIRF imaging for observing proteins on the cell surface.
- FRET and FLIM imaging for detecting protein interactions.
- Slide scanners for automated large scale tissue imaging in brightfield and fluorescence.
- Imaging of micro-fluidic devices.
- High-throughput screening of tissue and fluorescence in multi-well plates and dishes.
- Stereology for quantifying cell numbers and analysing axon tracks.
- Neurolucida and Imaris for neuron structure analysis and tracing.