Techniques used in the lab include

 

  • Magnetic Resonance Imaging – diffusion tensor imaging (human and animal).
  • In utero surgery, in utero electroporation of genetic constructs to label cells, over-express genes, or knock-down protein expression by siRNA.
  • Tissue culture and in vitro primary cultures, including organotypic slice culture.
  • Neuroanatomical and Histological approaches including tract tracing, immunohistochemistry, cryostat, vibratome, freezing microtome and electron and confocal microscopy
  • Molecular Biological approaches including protein purification, PCR, Northern, and Western Blotting, in situ hybridisation, chromatin immunoprecipitation, luciferase assays and genomic analysis