Lynch Publications
Islam, Robiul, Keramidas, Angelo, Xu, Li, Durisic, Nela, Sah, Pankaj and Lynch, Joseph W. (2016) Ivermectin-activated, cation-permeable glycine receptors for the chemogenetic control of neuronal excitation. ACS Chemical Neuroscience, 7 12: 1647-1657. doi:10.1021/acschemneuro.6b00168
Zhang, Yan, Keramidas, Angelo and Lynch, Joseph W. (2016) The free zinc concentration in the synaptic cleft of artificial glycinergic synapses rises to at least 1 µm. Frontiers in Molecular Neuroscience, 9 SEP2016: . doi:10.3389/fnmol.2016.00088
Zhang, Yan, Bode, Anna, Nguyen, Bindi, Angelo Keramidas and Lynch, Joseph W. (2016) Investigating the mechanism by which gain-of-function mutations to the a1 glycine receptor cause hyperekplexia. Journal of Biological Chemistry, 291 29: 15332-15341. doi:10.1074/jbc.M116.728592
Dixon, Christine L., Zhang, Yan and Lynch, Joseph W. (2015) Generation of functional inhibitory synapses incorporating defined combinations of GABA(A) or glycine receptor subunits. Frontiers in Molecular Neuroscience, 8 DEC: . doi:10.3389/fnmol.2015.00080
Cristofori-Armstrong, Ben, Soh, Ming S., Talwar, Sahil, Brown, Darren L., Griffin, John D. O., Dekan, Zoltan, Stow, Jennifer L., King, Glenn F., Lynch, Joseph W. and Rash, Lachlan D. (2015) Xenopus borealis as an alternative source of oocytes for biophysical and pharmacological studies of neuronal ion channels. Scientific Reports, 5 14763.1-14763.12. doi:10.1038/srep14763
Functional characterization of ivermectin binding sites in α1β2γ2L gaba(A) receptors
Estrada-Mondragon, Argel and Lynch, Joseph W. (2015) Functional characterization of ivermectin binding sites in α1β2γ2L gaba(A) receptors. Frontiers in Molecular Neuroscience, 8 September: . doi:10.3389/fnmol.2015.00055
Selective modulators of α5-containing GABAA receptors and their therapeutic significance
Soh, Ming Shiuan and Lynch, Joseph W. (2015) Selective modulators of α5-containing GABAA receptors and their therapeutic significance. Current Drug Targets, 16 7: 735-746. doi:10.2174/1389450116666150309120235
Benson, Melissa J, Thomas, Nicola K, Talwar, Sahil, Hodson, Mark P, Lynch, Joseph W, Woodruff, Trent M and Borges, Karin (2015) A novel anticonvulsant mechanism via inhibition of complement receptor C5ar1 in murine epilepsy models. Neurobiology of Disease, 76 87-97. doi:10.1016/j.nbd.2015.02.004
Investigating ion channel conformational changes using voltage clamp fluorometry
Talwar, Sahil and Lynch, Joseph W. (2015) Investigating ion channel conformational changes using voltage clamp fluorometry. Neuropharmacology, 98 3-12. doi:10.1016/j.neuropharm.2015.03.018
Correlating structural and energetic changes in glycine receptor activation
Scott, Suzanne, Lynch, Joseph W and Keramidas, Angelo (2015) Correlating structural and energetic changes in glycine receptor activation. Journal of Biological Chemistry, 290 9: 5621-5634. doi:10.1074/jbc.M114.616573
Zhang, Yan, Dixon, Christine L., Keramidas, Angelo and Lynch, Joseph W. (2015) Functional reconstitution of glycinergic synapses incorporating defined glycine receptor subunit combinations. Neuropharmacology, 89 391-397. doi:10.1016/j.neuropharm.2014.10.026
Zolpidem and eszopiclone prime α1β2γ2 GABAA receptors for longer duration of activity
Dixon, Christine L., Harrison, Neil L., Lynch, Joseph W. and Keramidas, Angelo (2015) Zolpidem and eszopiclone prime α1β2γ2 GABAA receptors for longer duration of activity. British Journal of Pharmacology, 172 14: 3522-3536. doi:10.1111/bph.13142
Ulens, Chris, Spurny, Radovan, Thompson, Andrew J., Alqazzaz, Mona, Debaveye, Sarah, Han, Lu, Price, Kerry, Villalgordo, Jose M., Tresadern, Gary, Lynch, Joseph W. and Lummis, Sarah C. R. (2014) The prokaryote ligand-gated ion channel ELIC captured in a pore blocker-bound conformation by the Alzheimer's disease drug memantine.Structure, 22 10: 1399-1407. doi:10.1016/j.str.2014.07.013
GABAa receptor α and γ subunits shape synaptic currents via different mechanisms
Dixon, Christine, Sah, Pankaj, Lynch, Joseph W. and Keramidas, Angelo (2014) GABAa receptor α and γ subunits shape synaptic currents via different mechanisms. Journal of Biological Chemistry, 289 9: 5399-5411. doi:10.1074/jbc.M113.514695
The impact of human hyperekplexia mutations on glycine receptor structure and function
Bode, Anna and Lynch, Joseph W. (2014) The impact of human hyperekplexia mutations on glycine receptor structure and function. Molecular Brain, 7 2: 1-12. doi:10.1186/1756-6606-7-2
Talwar, Sahil and Lynch, Joseph W. (2014) Phosphorylation mediated structural and functional changes in pentameric ligand-gated ion channels: Implications for drug discovery. International Journal of Biochemistry and Cell Biology, 53 218-233. doi:10.1016/j.biocel.2014.05.028
Bode, Anna and Lynch, Joseph W. (2013) Analysis of hyperekplexia mutations identifies transmembrane domain rearrangements that mediate glycine receptor activation. Journal of Biological Chemistry, 288 47: 33760-33771. doi:10.1074/jbc.M113.513804
Bode, Anna, Wood, Sian-Elin, Mullins, Jonathan G. L., Keramidas, Angelo, Cushion, Thomas D., Thomas, Rhys H., Pickrell, William O., Drew, Cheney J. G., Masri, Amira, Jones, Elizabeth A., Vassallo, Grace, Born, Alfred P., Alehan, Fusun, Aharoni, Sharon, Bannasch, Gerald, Bartsch, Marius, Kara, Bulent, Krause, Amanda, Karam, Elie G., Matta, Stephanie, Jain, Vivek, Mandel, Hanna, Freilinger, Michael, Graham, Gail E., Hobson, Emma, Chatfield, Sue, Vincent-Delorme, Catherine, Rahme, Jubran E., Afawi, Zaid, Berkovic, Samuel F., Howell, Owain W., Vanbellinghen, Jean-Francois, Rees, Mark I., Chung, Seo-Kyung and Lynch, Joseph W. (2013) New hyperekplexia mutations provide insight into glycine receptor assembly, trafficking, and activation mechanisms. Journal of Biological Chemistry, 288 47: 33745-33759. doi:10.1074/jbc.M113.509240
Phosphorylation of α3 glycine receptors induces a conformational change in the glycine-binding site
Han, Lu, Talwar, Sahil, Wang, Qian, Shan, Qiang and Lynch, Joseph W. (2013) Phosphorylation of α3 glycine receptors induces a conformational change in the glycine-binding site. ACS Chemical Neuroscience, 4 10: 1361-1370. doi:10.1021/cn400097j
Balansa, Walter, Islam, Robiul, Fontaine, Frank, Piggott, Andrew M., Zhang, Hua, Xiao, Xue, Webb, Timothy I., Gilbert, Daniel F., Lynch, Joseph W. and Capon, Robert J. (2013) Sesterterpene glycinyl-lactams: a new class of glycine receptor modulator from Australian marine sponges of the genus Psammocinia. Organic and Biomolecular Chemistry, 11 28: 4695-4701. doi:10.1039/c3ob40861b
Balansa, Walter, Islam, Robiul, Gilbert, Daniel F., Fontaine, Frank, Xiao, Xue, Zhang, Hua, Piggott, Andrew M., Lynch, Joseph W. and Capon, Robert J. (2013) Australian marine sponge alkaloids as a new class of glycine-gated chloride channel receptor modulator. Bioorganic and Medicinal Chemistry, 21 14: 4420-4425. doi:10.1016/j.bmc.2013.04.061
Novel missense mutations in the glycine receptor β subunit gene (GLRB) in startle disease
James, Victoria M., Bode, Anna, Chung, Seo-Kyung, Gill, Jennifer L., Nielsen, Maartje, Cowan, Frances M., Vujic, Mihailo, Thomas, Rhys H., Rees, Mark I., Harvey, Kirsten, Keramidas, Angelo, Topf, Maya, Ginjaar, Ieke, Lynch, Joseph W. and Harvey, Robert J. (2013) Novel missense mutations in the glycine receptor β subunit gene (GLRB) in startle disease. Neurobiology of Disease, 52 137-149. doi:10.1016/j.nbd.2012.12.001
Talwar, Sahil, Lynch, Joseph W. and Gilbert, Daniel F. (2013) Fluorescence-based high-throughput functional profiling of ligand-gated ion channels at the level of single cells. PLoS One, 8 3: e58479.1-e58479.11. doi:10.1371/journal.pone.0058479
GLRB is the third major gene of effect in hyperekplexia
Chung, Seo-Kyung, Bode, Anna, Cushion, Thomas D., Thomas, Rhys H., Hunt, Charlotte, Wood, Sian-Elin, Pickrell, William O., Drew, Cheney J. G., Yamashita, Sumimasa, Shiang, Rita, Leiz, Steffen, Longhardt, Ann-Carolyn, Raile, Vera, Weschke, Bernhard, Puri, Ratna D., Verma, Ishwar C., Harvey, Robert J., Ratnasinghe, Didi D., Parker, Michael, Rittey, Chris, Masri, Amira, Lingappa, Lokesh, Howell, Owain W., Vanbellinghen, Jean-François, Mullins, Jonathan G., Lynch, Joseph W. and Rees, Mark I. (2013) GLRB is the third major gene of effect in hyperekplexia. Human Molecular Genetics, 22 5: 927-940. doi:10.1093/hmg/dds498
The relative orientation of the TM3 and TM4 domains varies between α1 and α3 glycine receptors
Han, Lu, Talwar, Sahil and Lynch, Joseph W. (2013) The relative orientation of the TM3 and TM4 domains varies between α1 and α3 glycine receptors. ACS Chemical Neuroscience, 4 2: 248-254. doi:10.1021/cn300177g
An outline of desensitization in pentameric ligand-gated ion channel receptors
Keramidas, Angelo and Lynch, Joseph W. (2012) An outline of desensitization in pentameric ligand-gated ion channel receptors. Cellular and Molecular Life Sciences, 70 7: 1241-1253. doi:10.1007/s00018-012-1133-z
Ivermectin binding sites in human and invertebrate Cys-loop receptors
Lynagh, Timothy and Lynch, Joseph W. (2012) Ivermectin binding sites in human and invertebrate Cys-loop receptors. Trends in Pharmacological Sciences, 33 8: 432-441. doi:10.1016/j.tips.2012.05.002
Yang, Zhe, Taran, Elena, Webb, Timothy I. and Lynch, Joseph W. (2012) Stoichiometry and subunit arrangement of alpha 1 beta glycine receptors as determined by atomic force microscopy. Biochemistry, 51 26: 5229-5231. doi:10.1021/bi300063m
Molecular mechanisms of Cys-loop ion channel receptor modulation by ivermectin
Timothy Lynagh and Lynch, Joseph W. (2012) Molecular mechanisms of Cys-loop ion channel receptor modulation by ivermectin. Frontiers in Molecular Neuroscience, 5 60.1-60.11. doi:10.3389/fnmol.2012.00060
Shan, Qiang, Han, Lu and Lynch, Joseph W. (2012) Distinct properties of glycine receptor β+/α- interface: Unambiguously characterizing heteromeric interface reconstituted in homomeric protein. Journal of Biological Chemistry, 287 25: 21244-21252. doi:10.1074/jbc.M111.337741
Shan, Qiang, Han, Lu and Lynch, Joseph W. (2012) Function of hyperekplexia-causing alpha(1)R271Q/L glycine receptors is restored by shifting the affected residue out of the allosteric signaling pathway. British Journal of Pharmacology, 165 7: 2113-2123. doi:10.1111/j.1476-5381.2011.01701.x
Mechanism of action of the insecticides, lindane and fipronil, on glycine receptor chloride channels
Islam, Robiul and Lynch, Joseph W. (2012) Mechanism of action of the insecticides, lindane and fipronil, on glycine receptor chloride channels. British Journal of Pharmacology, 165 8: 2707-2720. doi:10.1111/j.1476-5381.2011.01722.x
Shan, Qiang and Lynch, Joseph W. (2012) Incompatibility between a pair of residues from the Pre-M1 linker and Cys-loop blocks surface expression of the Glycine Receptor. Journal of Biological Chemistry, 287 10: 7535-7542. doi:10.1074/jbc.M111.325126
Wang, Qian and Lynch, Joseph W. (2012) A comparison of glycine-and ivermectin-mediated conformational changes in the glycine receptor ligand-binding domain. International Journal of Biochemistry and Cell Biology, 44 2: 335-340. doi:10.1016/j.biocel.2011.11.005
Molecular determinants of ivermectin sensitivity at the glycine receptor chloride channel
Lynagh, Timothy, Webb, Timothy I., Dixon, Christine L., Cromer, Brett A. and Lynch, Joseph W. (2011) Molecular determinants of ivermectin sensitivity at the glycine receptor chloride channel. Journal of Biological Chemistry, 286 51: 43913-43924. doi:10.1074/jbc.M111.262634
Shan, Qiang, Han, Lu and Lynch, Joseph W. (2011) Beta subunit M2-M3 loop conformational changes are uncoupled from alpha1 beta glycine receptor channel gating: Implications for human hereditary hyperekplexia. PLoS One, 6 11: e28105.1-e28105.11. doi:10.1371/journal.pone.0028105
Wang, Qian and Lynch, Joseph W. (2011) Activation and desensitization induce distinct conformational changes at the extracellular-transmembrane domain interface of the glycine receptor. Journal of Biological Chemistry, 286 44: 38814-38824. doi:10.1074/jbc.M111.273631
Pless, Stephan A., Hanek, Ariele P., Price, Kerry L., Lynch, Joseph W., Lester, Henry A., Dougherty, Dennis A. and Lummis, Sarah C. R. (2011) A cation-π interaction at a phenylalanine residue in the glycine receptor binding site is conserved for different agonists. Molecular Pharmacology, 79 4: 742-748. doi:10.1124/mol.110.069583
Wang, Qian, Pless, Stephan A. and Lynch, Joseph W. (2010) Ligand- and subunit-specific conformational changes in the ligand-binding domain and the TM2-TM3 linker of α1 β2 γ2 GABAA receptors. Journal of Biological Chemistry, 285 51: 40373-40386. doi:10.1074/jbc.M110.161513
A glycine residue essential for high ivermectin sensitivity in Cys-loop ion channel receptors
Lynagh, Timothy and Lynch, Joseph W. (2010) A glycine residue essential for high ivermectin sensitivity in Cys-loop ion channel receptors. International Journal for Parasitology, 40 13: 1477-1481. doi:10.1016/j.ijpara.2010.07.010
Chimera construction using multiple-template-based sequential PCRs
Shan, Qiang and Lynch, Joseph W. (2010) Chimera construction using multiple-template-based sequential PCRs.Journal of Neuroscience Methods, 193 1: 86-89. doi:10.1016/j.jneumeth.2010.08.033
Gebhardt, FM, Mitrovic, AD, Gilbert, DF, Vandenberg, RJ, Lynch, JW and Dodd, PR (2010) Exon-skipping splice variants of excitatory amino acid transporter-2 (EAAT2) form heteromeric complexes with full-length EAAT2. Journal of Biological Chemistry, 285 41: 31313-31324. doi:10.1074/jbc.M110.153494
Pathophysiological mechanisms of dominant and recessive GLRA1 mutations in hyperekplexia
Chung, Seo-Kyung, Vanbellinghen, Jean-Francois, Mullins, Jonathan G. L., Robinson, Angela, Hantke, Janina, Hammond, Carrie L., Gilbert, Daniel F., Freilinger, Michael, Ryan, Monique, Kruer, Michael C., Masri, Amira, Gurses, Candan, Ferrie, Colin, Harvey, Kirsten, Shiang, Rita, Christodoulou, John, Andermann, Frederick, Andermann, Eva, Thomas, Rhy H., Harvey, Robert J., Lynch, Joseph W. and Rees, Mark I. (2010) Pathophysiological mechanisms of dominant and recessive GLRA1 mutations in hyperekplexia. Journal of Neuroscience, 30 28: 9612-9620. doi:10.1523/JNEUROSCI.1763-10.2010
Lynagh, Timothy and Lynch, Joseph W. (2010) An improved ivermectin-activated chloride channel receptor for inhibiting electrical activity in defined neuronal populations. Journal of Biological Chemistry, 285 20: 14890-14897. doi:10.1074/jbc.M110.107789
Balansa, Walter, Islam, Robiul, Fontaine, Frank, Piggott, Andrew M., Zhang, Hua, Webb, Timothy I., Gilbert, Daniel F., Lynch, Joseph W. and Capon, Robert J. (2010) Ircinialactams: Subunit-selective glycine receptor modulators from Australian sponges of the family Irciniidae. Bioorganic and Medicinal Chemistry, 18 8: 2912-2919. doi:10.1016/j.bmc.2010.03.002
Differential expression of glycine receptor subunits in the rat basolateral and central amygdala
Delaney, A. J., Esmaeili, A., Sedlak, Petra L., Lynch, J. W. and Sah, P. (2010) Differential expression of glycine receptor subunits in the rat basolateral and central amygdala. Neuroscience Letters, 469 2: 237-242. doi:10.1016/j.neulet.2009.12.003
Pless, Stephan A. and Lynch, Joseph W. (2009) Magnitude of a conformational change in the glycine receptor β1- β2 loop is correlated with agonist efficacy. The Journal of Biological Chemistry, 284 40: 27370-27376. doi:10.1074/jbc.M109.048405
Molecular determinants of β-carboline inhibition of the glycine receptor
Chen, Xuebin, Cromer, Brett. A. and Lynch, Joseph W. (2009) Molecular determinants of β-carboline inhibition of the glycine receptor. Journal Of Neurochemistry, 110 5: 1685-1694. doi:10.1111/j.1471-4159.2009.06273.x
Chen, Xuebin, Webb, Timothy I. and Lynch, Joseph W. (2009) The M4 transmembrane segment contributes to agonist efficacy differences between alpha1 and alpha3 glycine receptors. Molecular Membrane Biology, 26 5-7: 321-332. doi:10.1080/09687680903120319
Ligand-specific conformational changes in the α1 glycine receptor ligand-binding domain
Pless, Stephan A. and Lynch, Joseph W. (2009) Ligand-specific conformational changes in the α1 glycine receptor ligand-binding domain. The Journal of Biological Chemistry, 284 23: 15847-15856. doi:10.1074/jbc.M809343200
Gilbert, Daniel, Wilson, John C., Nink, Virginia, Lynch, Joseph W. and Osborne, Geoffrey W. (2009) Multiplexed labeling of viable cells for high-throughput analysis of glycine receptor function using flow cytometry. Cytometry Part A, 75A 5: 440-449. doi:10.1002/cyto.a.20703
Chen, Xuebin, Cromer, Brett, Webb, Timothy I., Yang, Zhe, Hantke, Janina, Harvey, Robert J., Parker, Michael W. and Lynch, Joseph W. (2009) Dihydropyridine inhibition of the glycine receptor: Subunit selectivity and a molecular determinant of inhibition. Neuropharmacology, 56 1: 318-327. doi:10.1016/j.neuropharm.2008.07.001
Pless, Stephan and Lynch, Joseph W. (2009) Distinct conformational changes in activated agonist-bound and agonist-free glycine receptor subunits. Journal Of Neurochemistry, 108 6: 1585-1594. doi:10.1111/j.1471-4159.2009.05930.x
Esmaeili, Abolghasem, Lynch, Joseph W. and Sah, Pankaj (2009) GABAᴀ receptors containing gamma 1 subunits contribute to inhibitory transmission in the central amygdala. Journal of Neurophysiology, 101 1: 341-349. doi:10.1152/jn.90991.2008
High throughput techniques for discovering new glycine receptor modulators and their binding sites
Gilbert, Daniel, Islam, Robiul, Lynagh, Timothy, Lynch, Joseph W. and Webb, Timothy I. (2009) High throughput techniques for discovering new glycine receptor modulators and their binding sites. Frontiers in Molecular Neuroscience, 2 OCT: 1-10. doi:10.3389/neuro.02.017.2009
Native glycine receptor subtypes and their physiological roles
Lynch, Joseph W. (2009) Native glycine receptor subtypes and their physiological roles. Neuropharmacology, 56 1: 303-309. doi:10.1016/j.neuropharm.2008.07.034
Gilbert, Daniel, Esmaeili, Abolghasem and Lynch, Joseph W. (2009) Optimizing the expression of recombinant αβγ GABAA receptors in HEK293 cells for high-throughput screening. Journal of Biomolecular Screening, 14 1: 86-91. doi:10.1177/1087057108328017
Pless, Stephan A., Millen, Kat S., Hanek, Ariele P., Lynch, Joseph W., Lester, Henry A., Lummis, Sarah C. R. and Dougherty, Dennis A. (2008) A Cation-π Interaction in the Binding Site of the Glycine Receptor Is Mediated by a Phenylalanine Residue. Journal of Neuroscience, 28 43: 10937-10942. doi:10.1523/JNEUROSCI.2540-08.2008
Subunit-specific modulation of glycine receptors by cannabinoids and N-arachidonyl-glycine
Yang, Zhe, Aubrey, Karin R., Alroy, Iris, Harvey, Robert J., Vandenberg, Robert J. and Lynch, Joseph W. (2008) Subunit-specific modulation of glycine receptors by cannabinoids and N-arachidonyl-glycine. Biochemical Pharmacology, 76 8: 1014-1023. doi:10.1016/j.bcp.2008.07.037
Illuminating the structure and function of Cys-loop receptors
Pless, Stephan A and Lynch, Joseph W (2008) Illuminating the structure and function of Cys-loop receptors. Clinical And Experimental Pharmacology And Physiology, 35 10: 1137-1142. doi:10.1111/j.1440-1681.2008.04954.x
Lynch, Joseph W. and Chen, Xuebin (2008) Subunit-specitic potentiation of recombinant glycine receptors by NV-31, a bilobalide-derived compound. Neuroscience letters, 435 2: 147-151. doi:10.1016/j.neulet.2008.02.022
Structure-activity analysis of ginkgolide binding in the glycine receptor pore
Heads, J. A., Hawthorne, R. L., Lynagh, T. and Lynch, J. W. (2008) Structure-activity analysis of ginkgolide binding in the glycine receptor pore. Journal of Neurochemistry, 105 4: 1418-1427. doi:10.1111/j.1471-4159.2008.05244.x
Yang, Zhe, Ney, Agnieszka, Cromer, Brett A., Ng, Hooi-Ling, Parker, Michael W. and Lynch, Joseph W. (2007) Tropisetron modulation of the glycine receptor: femtomolar potentiation and a molecular determinant of inhibition.Journal of Neurochemistry, 100 3: 758-769. doi:10.1111/j.1471-4159.2006.04242.x
A proposed structural basis for picrotoxinin and picrotin binding in the glycine receptor pore
Yang, Zhe, Cromer, Brett A., Harvey, Robert J., Parker, Michael W. and Lynch, Joseph W. (2007) A proposed structural basis for picrotoxinin and picrotin binding in the glycine receptor pore. Journal of Neurochemistry, 103 2: 580-589. doi:10.1111/j.1471-4159.2007.04850.x
Yang, Zhe, Webb, Timothy I. and Lynch, Joseph W. (2007) Closed-state cross-linking of adjacent beta 1 Subunits in alpha 1 beta 1 GABA(a) receptors via introduced 6 ' cysteines. Journal of Biological Chemistry, 282 23: 16803-16810. doi:10.1074/jbc.M611555200
Pless, Stephen A., Dibas, Mohammed I., Lester, Henry A. and Lynch, Joseph W. (2007) Conformational variability of the glycine receptor M2 domain in response to activation by different agonists. Journal of Biological Chemistry, 282 49: 36057-36067. doi:10.1074/jbc.M706468200
Molecular pharmacology of the glycine receptor chloride channel
Webb, TI and Lynch, JW (2007) Molecular pharmacology of the glycine receptor chloride channel. Current Pharmaceutical Design, 13 23: 2350-2367. doi:10.2174/138161207781368693
Glycine receptors: A new therapeutic target in pain pathways
Lynch, JW and Callister, RJ (2006) Glycine receptors: A new therapeutic target in pain pathways. Current Opinion In Investigational Drugs, 7 1: 48-53.
High-throughput screening of glycine and GAB(A)A receptors: Why and how?
Lynch, J. W. (2006) High-throughput screening of glycine and GAB(A)A receptors: Why and how?. Alcoholism-Clinical And Experimental Research, 30 9: 61A-61A.
Molecular determinants of ginkgolide binding in the glycine receptor pore
Hawthorne, R., Cromer, B. A., Ng, H. L., Parker, M. W. and Lynch, J. W. (2006) Molecular determinants of ginkgolide binding in the glycine receptor pore. Journal of Neurochemistry, 98 2: 395-407. doi:10.1111/j.1471-4159.2006.03875.x
Kruger, Wade, Gilbert, Daniel, Hawthorne, Rebecca, Hryciw, Deanne H., Frings, Stephan, Poronnik, Philip and Lynch, Joseph W. (2005) A yellow fluorescent protein-based assay for high-throughput screening of glycine and GABA(A) receptor chloride channels. Neuroscience Letters, 380 3: 340-345. doi:10.1016/j.neulet.2005.01.065
A picrotoxin-specific conformational change in the glycine receptor M2-M3 loop.
Hawthorne, Rebecca L. and Lynch, Joseph W. (2005) A picrotoxin-specific conformational change in the glycine receptor M2-M3 loop.. Journal of Biological Chemistry, 280 43: 35836-35843. doi:10.1074/jbc.M506645200
High throughput screening of neuronal Cl- channels: Why and how?
Lynch, J. W. (2005) High throughput screening of neuronal Cl- channels: Why and how?. Current Neuropharmacology, 3 4: 207-216. doi:10.2174/1570159054368286
Barry, P. H. and Lynch, J. W. (2005) Ligand-gated channels. Ieee Transactions On Nanobioscience, 4 1: 70-80. doi:10.1109/TNB.2004.842497
Molecular structure and function of the glycine receptor chloride channel
Lynch, J. W. (2004) Molecular structure and function of the glycine receptor chloride channel. Physiological Reviews,84 4: 1051-1095. doi:10.1152/physrev.00042.2003
Han, N. L. R., Clements, J. D. and Lynch, J. W. (2004) Comparison of taurine- and glycine-induced conformational changes in the M2-M3 domain of the glycine receptor. Journal of Biological Chemistry, 279 19: 19559-19565. doi:10.1074/jbc.M400548200
Asymmetric contribution of α and β subunits to the activation of αβ heteromeric glycine receptors
Shan, Q., Nevin, S. T., Haddrill, J. L. and Lynch, J. W. (2003) Asymmetric contribution of α and β subunits to the activation of αβ heteromeric glycine receptors. Journal of Neurochemistry, 86 2: 498-507. doi:10.1046/j.1471-4159.2003.01872.x
Insights into the structural basis for zinc inhibition of the glycine receptor
Nevin, S. T., Cromer, B. A., Haddrill, J. L., Morton, C. J., Parker, M. W. and Lynch, J. W. (2003) Insights into the structural basis for zinc inhibition of the glycine receptor. Journal of Biological Chemistry, 278 31 August 1: 28985-28992. doi:10.1074/jbc.M300097200
Shan, Qiang, Haddrill, Justine L. and Lynch, Joseph W. (2002) Comparative surface accessibility of a pore-lining threonine residue (T6') in the glycine and GABA(A) receptors. Journal of Biological Chemistry, 277 47: 44845-44853. doi:10.1074/jbc.M208647200
Seebungkert, B. and Lynch, J. W. (2002) Effects of polyunsaturated fatty acids on voltage-gated K+ and Na+ channels in rat olfactory receptor neurons. European Journal of Neuroscience, 16 8: 2085-2094. doi:10.1046/j.1460-9568.2002.02288.x
Han, Nian-Lin R., Haddrill, Justine L. and Lynch, Joseph W. (2001) Characterization of a glycine receptor domain that controls the binding and gating mechanisms of the beta-amino acid agonist, taurine. Journal of Neurochemistry, 79 3: 636-647. doi:10.1046/j.1471-4159.2001.00601.x
Ivermectin, an unconventional agonist of the glycine receptor chloride channel
Shan, Q., Haddrill, J. L. and Lynch, J. W. (2001) Ivermectin, an unconventional agonist of the glycine receptor chloride channel. Journal of Biological Chemistry, 276 16: 12556-12564. doi:10.1074/jbc.M011264200
Seebungkert, B and Lynch, JW (2001) A common inhibitory binding site for zinc and odorants at the voltage-gated K+ channel of rat olfactory receptor neurons. European Journal of Neuroscience, 14 2: 353-362. doi:10.1046/j.0953-816x.2001.01646.x
Shan, Q., Haddrill, J. L. and Lynch, J. W. (2001) A single beta subunit M2 domain residue controls the picrotoxin sensitivity of alpha beta heteromeric glycine receptor chloride channels. Journal of Neurochemistry, 76 4: 1109-1120. doi:10.1046/j.1471-4159.2001.00124.x
The surface accessibility of the glycine receptor M2-M3 loop is increased in the channel open state
Lynch, J. W., Han, N. L. R., Haddrill, J., Pierce, K. D. and Schofield, P. R. (2001) The surface accessibility of the glycine receptor M2-M3 loop is increased in the channel open state. Journal of Neuroscience, 21 8: 2589-2599.
Nevin, Simon T., Haddrill, Justine L. and Lynch, Joseph W. (2000) A pore-lining glutamic acid in the rat olfactory cyclic nucleotide-gated channel controls external spermine block. Neuroscience Letters, 296 2-3: 163-167. doi:10.1016/S0304-3940(00)01650-5
Rectification of the olfactory cyclic nucleotide-gated channel by intracellular polyamines
Lynch, J. W. (1999) Rectification of the olfactory cyclic nucleotide-gated channel by intracellular polyamines. Journal of Membrane Biology, 170 3: 213-227. doi:10.1007/s002329900551
Identification of a new ligand binding domain in the al subunit of the inhibitory glycine receptor
Vafa, B, Lewis, TM, Cunningham, AM, Jacques, P, Lynch, JW and Schofield, PR (1999) Identification of a new ligand binding domain in the al subunit of the inhibitory glycine receptor. Journal of Neurochemistry, 73 5: 2158-2166.
Hallani, M, Lynch, JW and Barry, PH (1998) Characterization of calcium-activated chloride channels in patches excised from the dendritic knob of mammalian olfactory receptor neurons. Journal of Membrane Biology, 161 2: 163-171. doi:10.1007/s002329900323
Nitric oxide inhibition of the rat olfactory cyclic nucleotide-gated cation channel
Lynch, JW (1998) Nitric oxide inhibition of the rat olfactory cyclic nucleotide-gated cation channel. Journal of Membrane Biology, 165 3: 227-234. doi:10.1007/s002329900436
Zinc potentiation of the glycine receptor chloride channel is mediated by allosteric pathways
Lynch, JW, Jacques, P, Pierce, KD and Schofield, PR (1998) Zinc potentiation of the glycine receptor chloride channel is mediated by allosteric pathways. Journal of Neurochemistry, 71 5: 2159-2168.
Balasubramanian, S, Lynch, JW and Barry, PH (1997) Concentration dependence of sodium permeation and sodium ion interactions in the cyclic AMP-gated channels of mammalian olfactory receptor neurons. Journal of Membrane Biology, 159 1: 41-52. doi:10.1007/s002329900267
Lynch, JW, Rajendra, S, Pierce, KD, Handford, CA, Barry, PH and Schofield, PR (1997) Identification of intracellular and extracellular domains mediating signal transduction in the inhibitory glycine receptor chloride channel. Embo Journal, 16 1: 110-120. doi:10.1093/emboj/16.1.110
Chiu, P, Lynch, JW and Barry, PH (1997) Odorant-induced currents in intact patches from rat olfactory receptor neurons: Theory and experiment. Biophysical Journal, 72 3: 1442-1457.
Rajendra, S, Lynch, JW and Schofield, PR (1997) The glycine receptor. Pharmacology & Therapeutics, 73 2: 121-146. doi:10.1016/S0163-7258(96)00163-5
Balasubramanian, S, Lynch, JW and Barry, PH (1996) Calcium-dependent modulation of the agonist affinity of the mammalian olfactory cyclic nucleotide-gated channel by calmodulin and a novel endogenous factor. Journal of Membrane Biology, 152 1: 13-23. doi:10.1007/s002329900081
Schofield, PR, Lynch, JW, Rajendra, S, Pierce, KD, Handford, CA and Barry, PH (1996) Molecular and genetic insights into ligand binding and signal transduction at the inhibitory glycine receptor. Cold Spring Harbor Symposia On Quantitative Biology, 61 333-342.
Handford, CA, Lynch, JW, Baker, E, Webb, GC, Ford, JH, Sutherland, GR and Schofield, PR (1996) The human glycine receptor beta subunit: Primary structure, functional characterisation and chromosomal localisation of the human and murine genes. Molecular Brain Research, 35 1-2: 211-219.
Balasubramanian, S, Lynch, JW and Barry, PH (1995) The Permeation of Organic Cations Through Camp-Gated Channels in Mammalian Olfactory Receptor Neurons. Journal of Membrane Biology, 146 2: 177-191.
Lynch, Joseph W., Rajendra, Sundran, Barry, Peter H. and Schofield, Peter R. (1995) Mutations affecting the glycine receptor agonist transduction mechanism convert the competitive antagonist, picrotoxin, into an allosteric potentiator. Journal of Biological Chemistry, 270 23: 13799-13806. doi:10.1074/jbc.270.23.13799
Rajendra, S, Lynch, JW, Pierce, KD, French, CR, Barry, PH and Schofield, PR (1995) Mutation of An Arginine Residue in the Human Glycine Receptor Transforms Beta-Alanine and Taurine From Agonists Into Competitive Antagonists.Neuron, 14 1: 169-175. doi:10.1016/0896-6273(95)90251-1
Startle disease mutations reduce the agonist sensitivity of the human inhibitory glycine receptor
Rajendra, Sundran, Lynch, Joseph W., Pierce, Kerrie D., French, Chris R., Barry, Peter H. and Schofield, Peter R. (1994) Startle disease mutations reduce the agonist sensitivity of the human inhibitory glycine receptor. Journal of Biological Chemistry, 269 29: 18739-18742.
Ryan, SG, Buckwalter, MS, Lynch, JW, Handford, CA, Segura, L, Shiang, R, Wasmuth, JJ, Camper, SA, Schofield, P and Oconnell, P (1994) A Missense Mutation in the Gene Encoding the Alpha(1) Subunit of the Inhibitory Glycine Receptor in the Spasmodic Mouse. Nature Genetics, 7 2: 131-135. doi:10.1038/ng0694-131
Lynch, Joseph W., Lemos, Virginia Soar, Bucher, Bernard, Stoclet, Jean-Claude and Takeda, Kenneth (1994) A pertussis toxin-insensitive calcium influx mediated by neuropeptide Y2 receptors in a human neuroblastoma cell line.Journal of Biological Chemistry, 269 11: 8226-8233.
Lynch, JW and Lindemann, B (1994) Cyclic Nucleotide-Gated Channels of Rat Olfactory Receptor-Cells - Divalent-Cations Control the Sensitivity to Camp. Journal of General Physiology, 103 1: 87-106. doi:10.1085/jgp.103.1.87
Frings, S, Lynch, JW and Lindemann, B (1992) Properties of Cyclic-Nucleotide Gated Channels Mediating Olfactory Transduction - Activation, Selectivity, and Blockage. Journal of General Physiology, 100 1: 45-67. doi:10.1085/jgp.100.1.45
An Analysis of Na+ Currents in Rat Olfactory Receptor Neurons
Rajendra, S, Lynch, JW and Barry, PH (1992) An Analysis of Na+ Currents in Rat Olfactory Receptor Neurons.Pflugers Archiv-European Journal of Physiology, 420 3-4: 342-346. doi:10.1007/BF00374468
Slowly Activating K+ Channels in Rat Olfactory Receptor Neurons
Lynch, JW and Barry, PH (1991) Slowly Activating K+ Channels in Rat Olfactory Receptor Neurons. Proceedings of the Royal Society B-Biological Sciences, 244 1311: 219-225. doi:10.1098/rspb.1991.0074
Lynch, JW and Barry, PH (1991) Properties of Transient K+ Currents and Underlying Single K+ Channels in Rat Olfactory Receptor Neurons. Journal of General Physiology, 97 5: 1043-1072. doi:10.1085/jgp.97.5.1043
Liquid Junction Potentials and Small-Cell Effects in Patch-Clamp Analysis
Barry, PH and Lynch, JW (1991) Liquid Junction Potentials and Small-Cell Effects in Patch-Clamp Analysis. Journal of Membrane Biology, 121 2: 101-117. doi:10.1007/BF01870526
Inward Rectification in Rat Olfactory Receptor Neurons
Lynch, JW and Barry, PH (1991) Inward Rectification in Rat Olfactory Receptor Neurons. Proceedings of the Royal Society B-Biological Sciences, 243 1307: 149-153. doi:10.1098/rspb.1991.0024
Action-Potentials Initiated by Single Channels Opening in a Small Neuron (Rat Olfactory Receptor)
Lynch, JW and Barry, PH (1989) Action-Potentials Initiated by Single Channels Opening in a Small Neuron (Rat Olfactory Receptor). Biophysical Journal, 55 4: 755-768.
Lynch, JW, Barry, PH and Quartararo, N (1988) A Temperature and Solution Control-System for the Measurement of Single Channel Currents in Excised Membrane Patches. Pflugers Archiv-European Journal of Physiology, 412 3: 322-327. doi:10.1007/BF00582515