Speaker : 

Professor Alice Pebay

Department of Surgery, Melbourne Medical School, Department of Anatomy and Neuroscience | School of Biomedical Sciences

The University of Melbourne, VIC
 

Title: "Modelling neurodegeneration using patient induced pluripotent stem cells"

Abstract: The difficulty in obtaining tissue from the eye or brain of living people is a major barrier to the understanding and development of new treatments for neurodegenerative diseases. Patient-specific induced pluripotent stem cells (iPSCs) provide a powerful means by which to investigate the pathogenesis of disease, as these cells can be differentiated into relevant cell types of interest for disease modelling, drug screening, and understanding of fundamental biology. Yet, for complex diseases, which involve various genetic and environmental factors, large numbers of samples are necessary to robustly confirm biological effects. To enable large-scale iPSC-disease modelling studies and standardisation of iPSC culture, we have established protocols for automation of stem cell culture that ensure sufficient throughput and standardisation of iPSC culture with minimal variation. Similarly, for monogenic conditions, that are amenable to gene-editing and thus require smaller sample size for disease modelling, we combine CRISPR/Cas9- editing with iPSCs, to generate isogenic iPSCs lines, allowing a robust in vitro model of disease.  Both large-scale and gene editing approaches will be illustrated here.
 
 

 

 

 

About Neuroscience Seminars

Neuroscience seminars at the QBI play a major role in the advancement of neuroscience in the Asia-Pacific region. The primary goal of these seminars is to promote excellence in neuroscience through the exchange of ideas, establishing new collaborations and augmenting partnerships already in place.

The scheduled QBI Neuroscience Seminar series are held on Wednesdays from 11am-12pm in the Level 7 Auditorium of the Queensland Brain Institute, Building 79, St Lucia Campus, The University of Queensland. Additional seminars may be held at other times as listed below.

 

Neuroscience Seminars archive 2005-2018