Dr Christian Winterflood, Zurich : "Applications of supercritical angle and single-molecule fluorescence''
Speaker:
Dr Christian Winterflood, Zurich, Switzerland
Title: "Applications of supercritical angle and single-molecule fluorescence"
Abstract:
Highly surface-confined and efficient fluorescence detection can be achieved by the detection of near-field mediated supercritical angle fluorescence (SAF). This approach allows for undercritical angle fluorescence to be detected from deeper axial planes simultaneously. I will present applications of SAF detection ranging from sub-diffraction imaging to biosensing. I will also present a straightforward method for two-colour 3D dSTORM. The combination of biplane imaging and spectral demixing with two highly performant dyes eliminates several problems, including crosstalk, chromatic aberration or the need for colour registration. Lastly, I will present an investigation of the diffusion barrier to the lateral exchange of molecules in the axon initial segment membrane of neurons by correlative single-particle tracking and dSTORM, revealing a highly ordered nanoscopic compartmentalisation.
About Neuroscience Seminars
Neuroscience seminars at the QBI play a major role in the advancement of neuroscience in the Asia-Pacific region. The primary goal of these seminars is to promote excellence in neuroscience through the exchange of ideas, establishing new collaborations and augmenting partnerships already in place.
Seminars in the QBI Auditorium on Level 7 are held on Wednesdays at 12-1pm, which are sometimes simulcast on Zoom (with approval from the speaker). We also occassionally hold seminars from international speakers via Zoom. The days and times of these seminars will vary depending on the time zone of the speaker. Please see each seminar listed below for details.
