Advanced Microscopy Facility
Services and support
- Information and advice on imaging techniques and experiment design
- Instrument training: from basic use through to advanced techniques
- Training in post-acquisition analysis – including Imaris, Amira, ilastik, Neurolucida, CellProler, deconvolution, Image/FIJI
- Ongoing tailored advice and support for imaging projects
Systems and equipment
Super-resolution Microscopes Lightsheet Microscopes Confocal Microscopes Stereology Microscopes TIRF Microscopes Analysis Workstations Epifluorescence Microscopes USB3.0 Camera Slide Scanner Microscopes
Super-resolution Microscopes
- Roper iLas 2 Microscope (Level 5 Imaging Suite) User guide
- ELYRA SIM/PALM/STORM Microscope (Level 5 Imaging Suite) User guide
Confocal Microscopes
- LSM 510 META (Level 5 Imaging Suite) User guide
- LSM 710 2photon + BiG Detectors (Level 5 Imaging Suite) User guide
- Diskovery Spinning Disk Confocal (412) User guide
- Yokogawa W1 Spinning Disk Confocal (412) User guide
TIRF Microscopes
- Marianas TIRF FRET FLIM FRAP (Level 5 Imaging Suite) User guide
Epifluorescence Microscopes
- Axio Imager Green (414) User guide
- Axio Imager Blue (616) User guide
- Axio Imager Azure (414) User guide
- Axio Observer Indigo (618) - Inverted User guide
- Axio Observer Yellow (CRISP) (Ritchie 3b) - Inverted User guide
- Nikon Ti Eclipse Amber (Ritchie 3b) - Inverted User guide
- StereoDiscovery V8 Magenta (618) User guide
Slide Scanner Microscopes
- Brighteld Slide Scanner (414) User guide
- Fluorescence Slide Scanner (414) User guide
Lightsheet Microscopes
- 2-photon Scanned Lightsheet Microscope (513a) User guide
Stereology Microscopes
- Stereology Microscope (618) - Inverted User guide
Analysis Workstations
- Workstation 1: Imaris / Super-resolution (616) User guide
- Workstation 2: Imaris / Confocal Analysis (616) User guide
- Workstation 3: Neurolucida/Stereology (Level 7) User guide
USB3.0 Camera
- Thorlabs USB3 Camera (534) User guide
Technical capabilities
- Two-photon/single-photon scanned light-sheet microscopy
- Bright-eld and multi-channel uorescence microscopy
- Laser scanning confocal microscopy, including spectral imaging and multi-positional imaging
- Rapid spinning disk confocal imaging for experiments in tissue and cells
- Super-resolution imaging using single-molecule localisation and structured illumination
- Two-photon and confocal microscopy for tissue imaging and nano-scissor experiments
- Long-term (48+ hrs) live imaging in bright-eld and uorescence
- Rapid-acquisition live imaging for intra-cellular tracking and calcium imaging
- TIRF microscopy for observing proteins on the cell membrane
- FRET and FLIM imaging for detecting protein interactions
- Slide scanners for automated large-scale tissue imaging in bright-eld and uorescence
- Imaging of micro-uidic devices
- High-throughput screening of tissue and uorescence in multi-well plates and dishes
- Stereology for quantifying cell numbers and analysing axon tracks
- Neurolucida, Imaris and Amira for neuron structure analysis and tracing and image processing and segmentation