Queensland Brain Institute

Locomotor Circuits in Drosophila

  • Professor Ansgar Büschges, University of Cologne, Germany
  • Professor Richard Mann, Columbia University, New York
  • Professor Silvia Daun-Gruhn, University of Cologne, Cermany
  • Dr Gwyneth Card, Janelia Research Campus, HHMI, U.S.A.
  • Dr Julie Simpson, Janelia Research Campus, HHMI, U.S.A.

  •  A rise-to-threshold process for a relative-value decision

    Vijayan, Vikram, Wang, Fei, Wang, Kaiyu, Chakravorty, Arun, Adachi, Atsuko, Akhlaghpour, Hessameddin, Dickson, Barry J. and Maimon, Gaby (2023). A rise-to-threshold process for a relative-value decision. Nature, 619 (7970), 1-9. doi: 10.1038/s41586-023-06271-6

  • Ascending neurons convey behavioral state to integrative sensory and action selection brain regions

    Chen, Chin-Lin, Aymanns, Florian, Minegishi, Ryo, Matsuda, Victor D. V., Talabot, Nicolas, Günel, Semih, Dickson, Barry J. and Ramdya, Pavan (2023). Ascending neurons convey behavioral state to integrative sensory and action selection brain regions. Nature Neuroscience, 26 (4), 682-695. doi: 10.1038/s41593-023-01281-z

  • A searchable image resource of Drosophila GAL4-driver expression patterns with single neuron resolution

    Meissner, Geoffrey Wilson, Nern, Aljoscha, Dorman, Zachary, DePasquale, Gina M, Forster, Kaitlyn, Gibney, Theresa, Hausenfluck, Joanna H, He, Yisheng, Iyer, Nirmala A, Jeter, Jennifer, Johnson, Lauren, Johnston, Rebecca M, Lee, Kelley, Melton, Brian, Yarbrough, Brianna, Zugates, Christopher T, Clements, Jody, Goina, Cristian, Otsuna, Hideo, Rokicki, Konrad, Svirskas, Robert R, Aso, Yoshinori, Card, Gwyneth M, Dickson, Barry J, Ehrhardt, Erica, Goldammer, Jens, Ito, Masayoshi, Kainmueller, Dagmar, Korff, Wyatt ... Malkesman, Oz (2023). A searchable image resource of Drosophila GAL4-driver expression patterns with single neuron resolution. eLife, 12. doi: 10.7554/elife.80660

  • Rapid reconstruction of neural circuits using tissue expansion and light sheet microscopy

    Lillvis, Joshua L., Otsuna, Hideo, Ding, Xiaoyu, Pisarev, Igor, Kawase, Takashi, Colonell, Jennifer, Rokicki, Konrad, Goina, Cristian, Gao, Ruixuan, Hu, Amy, Wang, Kaiyu, Bogovic, John, Milkie, Daniel E., Meienberg, Linus, Mensh, Brett D., Boyden, Edward S., Saalfeld, Stephan, Tillberg, Paul W. and Dickson, Barry J. (2022). Rapid reconstruction of neural circuits using tissue expansion and light sheet microscopy. eLife, 11, 1-36. doi: 10.7554/elife.81248

  • Neural network organization for courtship-song feature detection in Drosophila

    Baker, Christa A., McKellar, Claire, Pang, Rich, Nern, Aljoscha, Dorkenwald, Sven, Pacheco, Diego A., Eckstein, Nils, Funke, Jan, Dickson, Barry J. and Murthy, Mala (2022). Neural network organization for courtship-song feature detection in Drosophila. Current Biology, 32 (15), 3317-3333.e7. doi: 10.1016/j.cub.2022.06.019

  • Taste quality and hunger interactions in a feeding sensorimotor circuit

    Shiu, Philip K., Sterne, Gabriella R., Engert, Stefanie, Dickson, Barry J. and Scott, Kristin (2022). Taste quality and hunger interactions in a feeding sensorimotor circuit. eLife, 11 e79887, 1-24. doi: 10.7554/elife.79887

   Prof Barry Dickson or Dr Kai Feng

Project: Neural Circuits, Genetics and Behaviour

As animals walk, run, or hop, motor circuits in the spinal cord convert descending “command” signals from the brain into the coordinated movements of many different leg muscles. How are command signals from the brain deconvolved into the appropriate patterns of motor neuron activity? We aim to answer this question for Drosophila by studying the functional organization of leg motor circuits in the ventral nerve cord, the fly’s analogue of the spinal cord. In Drosophila, individual neuronal cell types can be reproducibly identified and manipulated using genetic reagents that have been developed to target specific descending neurons, interneurons, or motor neurons. We have also established imaging pipeline to identify novel neurons that are behaviourally relevant and probe how they talk to each other. A range of projects involving optogenetics, two-photon imaging, machine learning assisted behavioural analysis and circuit modelling are currently open to honours students with a background in any area of molecular biology or experimental or theoretical neuroscience.

 

How to apply

Project 1: Neural Mechanisms of Drosophila locomotion

Background

As animals walk, run, or hop, motor circuits in the spinal cord convert descending “command” signals from the brain into the coordinated movements of many different leg muscles. How are command signals from the brain deconvolved into the appropriate patterns for motor neuron activity?

Project aim

We aim to answer this question for Drosophila by studying the functional organization of leg motor circuits in the ventral nerve cord, the fly’s analogue of the spinal cord. In Drosophila, individual neuronal cell types can be reproducibly identified and manipulated using genetic reagents that have been developed to target specific descending neurons, interneurons, or motor neurons.

Your role

In your thesis project, you will learn a range of methods including genetics, multiphoton imaging, optogenetics and quantitative behavioural analysis, and use these methods to elucidate the structure and function of the motor circuits controlled by a specific class of descending neuron. This may be, for example, a descending neuron that, when activated, causes the fly to walk backwards (see Bidaye et al, Science 6179:97), or one that elicits turning. Understanding the circuit mechanisms behind those simple actions will shed light on general computational principles of neural networks and may even help us to design smarter robots.

How to apply 

Find out about the entry requirements, application procedures, and scholarship information and deadlines.

How to apply

Contact

Contact Professor Barry Dickson via b.dickson@uq.edu.au