Slide Scanner Microscopes: Fluorescence Slide Scanner (414)
Upright fluorescence slide scanner. Ideal for imaging large numbers of tissue sections.
Instrument:
Metafer VSlide Scanner by MetaSystems using Zeiss Axio Imager Z2.
Company website: http://www.metasystems-international.com/metafer/vslide
Features Include:
- Fully motorised X-Y-Z stage
- Z-stacks
- Tiled imaging
- Automated image capture experiments. Locates tissue with 5x objective, determines area to be scanned autonomously and generates focus map. Manual placement of focus points is not necessary.
- Automated robotic arm for loading slides – up to 80 slides per run.
Objectives:
Air:
- 2.5x 0.075NA / 9.5mm,
- 5x 0.15 NA / 13.6mm,
- 10x 0.3 NA / 5.5mm,
- 20x 0.8 NA / 0.55mm
Fluorescence Filter Sets (for checking sample with oculars):
- DAPI
- GFP / Alexa 488 (530/50)
- Cy3 / Alexa 546/555/568 (620/60)
- Cy5 / Alexa 633/647
Images produced by this scanner are full-resolution and uncompressed.
Please see the example movies and images below:
Example of triple labelled 50 µm mouse brain sections (Ilan Gobius – Richards Lab)
http://www.youtube.com/watch?v=qKAvPz3udRE&list=PL7026C3C5A9283C94
http://www.youtube.com/watch?v=0x_YUIJ-Zlc&list=PLA1C3C347B467C3E6