Upright fluorescent microscope fitted with both monochrome and colour cameras. Suited for fluorescence and bright field imaging of fixed samples on slides.

Features Include:

  • Fully motorised X-Y-Z stage
  • Z-stacks
  • Mosaix tiling
  • Mark and Find
  • Fully automated image capture experiments
  • ApoTome optical sectioning
  • DIC

Objectives:

  • Air:
    • 5x 0.16 NA / 18.5mm WD / 1.29 μm/pixel,
    • 10x  0.45 NA / 2mm WD / 0.645 μm/pixel,
    • 20x 0.8 NA / 0.55mm WD / 0.323 μm/pixel,
    • 40x 0.75 NA / 0.71mm WD /0.161 μm/pixel
  • Oil:
    • 63x 1.4 NA / 0.19mm WD / 0.102 μm/pixel
    • 100x 1.3 NA / 0.2mm WD / 0.065 μm/pixel

Fluorescent Filter Sets For:

  • DAPI (FS#49) BP335-383/BS395/BP 420-470
  • GFP / Alexa 488 (FS#44) BP455-495/BS500/BP505-555
  • Cy3 / Alexa 546/555/568 (FS#43) BP533-558/BS570/BP570-640
  • mCherry / Alexa 594 (FS#43) BP533-558/BS570/BP570-640
  • BFP / GFP / HcRed (FS#62HE)
    • BP450-390/BS395/BP402-448
    • BP460-488/BS495/BP500-557
    • BP567-602/BS610/LP615
  • Cy5 / Alexa 633/647 (FS#50) BP625-655/BS660/BP665-715
  • CFP (FS#47) BP426-446/BS455/ BP460-500
  • YFP (FS#46) BP490-510/BS515/BP520-550
  •  

Fluorescent Illumination:

  • Xenon HXP
  • Colibri LEDs – 365nm (DAPI), 470nm (GFP), 590nm (mCherry/Alexa594), 625nm (Cy5/Alexa647).

Illumination Power:

  • Xenon HXP: 405nm (3.99mW), 488nm (12.34mW), 568nm (14.22mW), 647nm (4.51mW)

Instructions

Guide to using Axio Imagers and Observers