An inverted spinning disk utilizing the W1 Yokogawa spinning disk module designed for large-field and tissue imaging.

The W1 module was installed by Intelligent Imaging Innovations on a Zeiss Axio Observer Z1 and is controlled by Slidebook 6.0 software.
A Photometrics Evolve EMCCD camera is used for rapid, low-signal imaging and a Hamamatsu Flash4.0 sCMOS for large-field and tissue imaging.

This microscope is equipped with several high-end objectives suitable for imaging live cells and model organisms as well as fixed cells and tissues.

Features:

  • Equipped with Yokogawa W1 disk head.csuw-small
  • Photometrics EMCCD for high-speed, low-light level imaging.
  • Hamamatsu Flash4.0 scientific CMOS camera with 2048×2048 chip enabling fast capture of large mosaic images.
  • Piezo z-drive and definite focus for rapid z-stack capture and focus stablisation.

Objectives:

Air:

  • 10x 0.45 NA / Plan-Apochromat / 520µm WD
  • 20x 0.8 NA / Plan-Apochromat / 550µm WD

Water:

  • 40x 1.2 NA / C-Apochromat / 280µm WD
  • 63x 1.2 NA / C-Apochromat / 280µm WD

Oil:

  • 63x 1.4 NA / Plan-Apochromat / 180µm WD
  • 100x 1.4 NA / Plan-Apochromat / 140µm WD

Lasers:

  • 405nm
  • 488nm
  • 561nm
  • 640nm

Cameras:

  • Hamamatsu ORCA-Flash4.0 V2 sCMOS (photon conversion factor 0.46)
  • photometrics Evolve EMCCD

When including this microscope in your methods:

” Cells/Tissue imaged on a spinning-disk confocal system (Marianas; 3I, Inc.) consisting of a Axio Observer Z1 (Carl Zeiss) equipped with a CSU-W1 spinning-disk head (Yokogawa Corporation of America), ORCA-Flash4.0 v2 sCMOS camera (Hamamatsu Photonics), 20x 0.8 NA PlanApo and 40x 1.2 NA C-Apo objectives. Image acquisition was performed using SlideBook 6.0 (3I, Inc).”

Guide to using the Spinning Disk Confocal