Inverted point-scanning laser confocal microscope with spectral detection and high-sensitivity BiG (GaAsP) detectors. 2-photon imaging with Mai Tai eHP 690-1040nm laser with dedicated BiG(GaAsP) detectors. Suited for imaging fixed samples on slides, samples with low fluorescence and live imaging.

Features Include:

  • Fully motorised X-Y-Z stage
  • Piezo Z-drive for rapid Z-stacks
  • High-sensitivity BiG detectors (GFP/mCherry) for both vis and 2-photon imaging
  • CO2 and temperature incubation for live cells and tissue
  • Tiled imaging
  • Multi-position imaging
  • Spectral imaging

Objectives:

  • Air:
    • 10x 0.45 NA/ 2000µm WD / 0.439 μm/pixel (1024×1024),
    • 20x 0.8 NA / 550µm WD / 0.415 µm/pixel (1024×1024),
  •  Water:
    • 32x 0.85 NA / 1100µm WD /0.259 µm/pixel (1024×1024),
    • 40x 1.2 NA / 280µm WD / 0.208 µm/pixel (1024×1024),
  •  Oil:
    • 40x 1.4 NA /  130µm WD / 0.208 µm/pixel (1024×1024),
    • 63x 1.4 NA / 190µm WD / 0.132 µm/pixel (1024×1024)

Fluorescent Filter Sets (for checking sample with oculars):

  • DAPI
  • GFP / Alexa 488
  • Cy3 / Alexa 546/555/568

Lasers:

  • Blue laser diode (405nm)
    • DAPI
  • Argon laser (458/477/488/514nm)
    • GFP / YFP / FITC / Alexa488
  • 561nm
    • Cy3 / Alexa 546/555/568 / Alexa 594 / mCherry
  • 633nm
    • Cy5 / Alexa647
  • Mai Tai eHP 2-photon laser / 760 – 1040nm

Laser Power:

Measurement at BFP

  • 405nm (4.65mW),
  • 458nm (0.1mW),
  • 488nm (0.821mW),
  • 514nm (0.178mW),
  • 561nm (3.919mW),
  • 633nm (1.119mW)
  • Mai-Tai @ 780nm (24.5mW)

Instructions

Guide to using the LSM 710