Mr Geoff Osborne - Director of Flow Cytometry

Mr Geoff Osborne

 Contact Information

 g.osborne@uq.edu.au
  Building: QBI Building #79
  Room: 532
  Tel: +61 7 334 66397

 Mailing Address

  Queensland Brain Institute
  The University of Queensland
  Brisbane, 4072
  Queensland,
  Australia

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Short biography

Research directions

Current collaborations

Selected publications

Short biography

Mr Osborne obtained an Associate Diploma in Applied Biology in 1983, before a Bachelor of Science from the Australian National University (ANU). He began specializing in flow cytometry in 1989 at the John Curtin School of Medical Research, ANU. From 1989 to 1999 his interest lay in developing novel flow cytometry technologies in software and hardware with a particular emphasis cell sorting and high content screening applications. In 2000 he moved to the University of British Columbia, Vancouver, Canada to establish a core facility before return to ANU in 2001. He was recruited in 2004 to a joint position at The Queensland Brain Institute and the Australian Institute for Bioengineering and Nanotechnology.

 
 

Research directions
 
Flow cytometry is a cornerstone technology for the study of haematopoietic stem cell function and cellular immunology in general. This is due not only to its ability to analyse and sort specific populations of stem, progenitor and more differentiated blood cell types, but also because it can provide quantitative information pertaining to viability, DNA content, mitotic activity and protein expression patterns of specific cell types. Given these strengths it is surprising that flow cytometry has previously had little traction in neuroscience. This laboratory seeks to combine these strengths with recent advances that allow the purification of essentially pure populations of viable neural stem cells, neural crest cells and post-mitotic neurons using flow cytometry, to explore new avenues for research.

Currently Mr Osborne’s research interests involve novel implementations of flow technology in neuroscience, in particular the role of flow cytometry can play in elucidating the biology of brain tumours and stem cells.


 
Current collaborations
 
Selected publications
 
Recent advances in flow cytometric cell sorting.
 GW Osborne
Methods Cell Biol, Jan 2011; 102: 533-56.

Purification of immature neuronal cells from neural stem cell progeny.
 H Azari, GW Osborne, T Yasuda, MG Golmohammadi, M Rahman, LP Deleyrolle, E Esfandiari, DJ Adams, B Scheffler, DA Steindler, and BA Reynolds PLoS One, January 1, 2011; 6(6): e20941.

Evidence for label-retaining tumour-initiating cells in human glioblastoma.
 Deleyrolle LP, Harding A, Cato K, Siebzehnrubl FA, Rahman M, Azari H, Olson S, Gabrielli B, Osborne G, Vescovi A, Reynolds BA (2011 Brain May 2011; 134: 1331 - 1343.

A method of quantifying cell sorting yield in "real time". Osborne G (2010) Cytometry A 77:983-989.

Cellular plasticity of inflammatory myeloid cells in the peritoneal foreign body response. Mooney J, Rolfe B, Osborne G, Sester D, van Rooijen N, Campbell G, Hume D, Campbell J (2010) Am J Pathol 176:369-380.
 

 

 


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